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cfx96 touch real time pcr detection system device  (Bio-Rad)


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    Structured Review

    Bio-Rad cfx96 touch real time pcr detection system device
    Cfx96 Touch Real Time Pcr Detection System Device, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 20008 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx96+device/CFX96+Touch/pm42105931-48-26-33
    Average 99 stars, based on 20008 article reviews
    cfx96 touch real time pcr detection system device - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Neuroprotective and Neurite Outgrowth Stimulating Effects of New Low-Basicity 5-HT 7 Receptor Agonists: In Vitro Study in Human Neuroblastoma SH-SY5Y Cells.
    Article Snippet: The concentration of RNA was measured using NanoDrop ND-1000 Spectrometer (NanoDrop Technologies Inc., Montchanin, DE, USA). .. Reverse transcription was performed with 2 μg RNA and NG dART RT Kit (EURx) according to manufacturer recommendation at 37 °C for 60 min. qPCR reaction was performed by use of TaqMan Assay-On-Demand probes (HTR7- Hs04194798_s1, HPRT-1 Hs02800695) and Fast Start Universal Probe Master (Roche) kit, according to the recommendations of the manufacturer, using CFX96 device (BioRad). cDNAs were diluted 1:10 with pharmaceutical purified water and approximately 90 ng of the cDNA synthesized from the RNA template from an individual sample was used for each reaction. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Neuroprotective and Neurite Outgrowth Stimulating Effects of New Low-Basicity 5-HT 7 Receptor Agonists: In Vitro Study in Human Neuroblastoma SH-SY5Y Cells.
    Article Snippet: The concentration of RNA was measured using NanoDrop ND-1000 Spectrometer (NanoDrop Technologies Inc., Montchanin, DE, USA). .. Reverse transcription was performed with 2 μg RNA and NG dART RT Kit (EURx) according to manufacturer recommendation at 37 °C for 60 min. qPCR reaction was performed by use of TaqMan Assay-On-Demand probes (HTR7- Hs04194798_s1, HPRT-1 Hs02800695) and Fast Start Universal Probe Master (Roche) kit, according to the recommendations of the manufacturer, using CFX96 device (BioRad). cDNAs were diluted 1:10 with pharmaceutical purified water and approximately 90 ng of the cDNA synthesized from the RNA template from an individual sample was used for each reaction. ..

    Article Title: HB-EGF activates EGFR to induce reactive neural stem cells in the mouse hippocampus after seizures
    Article Snippet: Reverse transcription was done using a high-capacity reverse transcription kit (Cat#S7810; Thermo Fisher Scientific). .. A real-time quantitative polymerase chain reaction (qRT–PCR) was performed on a Bio-Rad CFX96 device (Bio-Rad), using the following primers: Hypoxanthine–guanine phosphoribosyltransferase (HPRT) forward: 5′-GTT GGG CTT ACC TCA CTG CT-3′, reverse: 5′-TCA TCG CTA ATC ACG ACG CT-3′; glyceraldehyde phosphate dehydrogenase (GAPDH) forward: 5′-CCA GTA TGA CTC CAC TCA CG-3′, reverse: 5′-GAC TCC ACG ACA TAC TCA GC-3′; EGFR forward: 5′-GCC AAC TGT ACC TAT GGA TGT-3′, reverse: 5′-GGC CCA GAG GAT TTG GAA GAA-3′; FGFR 1 forward: 5′-CCA AAC CCT GTA GCT CCC TA-3′, reverse: 5′-TGA ACT TCA CCG TCT TGG CA-3′; FGFR2 forward: 5′-CCG AAT GAAGAC CAC GAC CA-3′; reverse: 5′-TCG GCC GAA ACT GTT ACC TG-3′; metallothionein (MTH) 1 forward: 5′-TCA CCA CGA CTT CAA CGT CC-3′, reverse: 5′-CAG TTG GGG TCC ATT CCG AG-3′; MTH2 forward: 5′-GCA TCT GCA AAG AGG CTT CC-3′, reverse: 5′-AGT TGT GGA GAA CGA GTC AGG-3′; MTH3 forward: 5′-GCT GCT GGA CTG GAT ATG GA-3′; reverse: 5′-TTG CAT TTG TCC GAG CAG GT-3′. ..

    Article Title: ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells
    Article Snippet: RNA was extracted with Tri-Reagent (Sigma) and reverse transcribed to cDNA with High-Capacity cDNA Reverse Transcription Kit (Life Technologies). .. RNA levels were measured by qPCR with iTaq Universal SYBR Green Supermix (BIO-RAD) and the BioRad CFX96 device. ..

    Purification:

    Article Title: Neuroprotective and Neurite Outgrowth Stimulating Effects of New Low-Basicity 5-HT 7 Receptor Agonists: In Vitro Study in Human Neuroblastoma SH-SY5Y Cells.
    Article Snippet: The concentration of RNA was measured using NanoDrop ND-1000 Spectrometer (NanoDrop Technologies Inc., Montchanin, DE, USA). .. Reverse transcription was performed with 2 μg RNA and NG dART RT Kit (EURx) according to manufacturer recommendation at 37 °C for 60 min. qPCR reaction was performed by use of TaqMan Assay-On-Demand probes (HTR7- Hs04194798_s1, HPRT-1 Hs02800695) and Fast Start Universal Probe Master (Roche) kit, according to the recommendations of the manufacturer, using CFX96 device (BioRad). cDNAs were diluted 1:10 with pharmaceutical purified water and approximately 90 ng of the cDNA synthesized from the RNA template from an individual sample was used for each reaction. ..

    Synthesized:

    Article Title: Neuroprotective and Neurite Outgrowth Stimulating Effects of New Low-Basicity 5-HT 7 Receptor Agonists: In Vitro Study in Human Neuroblastoma SH-SY5Y Cells.
    Article Snippet: The concentration of RNA was measured using NanoDrop ND-1000 Spectrometer (NanoDrop Technologies Inc., Montchanin, DE, USA). .. Reverse transcription was performed with 2 μg RNA and NG dART RT Kit (EURx) according to manufacturer recommendation at 37 °C for 60 min. qPCR reaction was performed by use of TaqMan Assay-On-Demand probes (HTR7- Hs04194798_s1, HPRT-1 Hs02800695) and Fast Start Universal Probe Master (Roche) kit, according to the recommendations of the manufacturer, using CFX96 device (BioRad). cDNAs were diluted 1:10 with pharmaceutical purified water and approximately 90 ng of the cDNA synthesized from the RNA template from an individual sample was used for each reaction. ..

    Isolation:

    Article Title: Integrated mathematical and experimental modeling uncovers enhanced EMT plasticity upon loss of the DLC1 tumor suppressor.
    Article Snippet: .. Total RNA isolation was performed using NucleoSpin RNA Kit (Macherey-Nagel), following the manufacturer’s protocol. qRT-PCR with 100 ng template total RNA per reaction was performed using the Cfx96 device (Bio-Rad), and the Power SYBR Green 1-Step kit (Thermo Fisher Scientific) according to manufacturer’s protocol. ..

    Article Title: Integrated mathematical and experimental modeling uncovers enhanced EMT plasticity upon loss of the DLC1 tumor suppressor
    Article Snippet: .. Total RNA isolation was performed using NucleoSpin RNA Kit (Macherey-Nagel), following the manufacturer’s protocol. qRT-PCR with 100 ng template total RNA per reaction was performed using the Cfx96 device (Bio-Rad), and the Power SYBR Green 1-Step kit (Thermo Fisher Scientific) according to manufacturer’s protocol. ..

    Quantitative RT-PCR:

    Article Title: Integrated mathematical and experimental modeling uncovers enhanced EMT plasticity upon loss of the DLC1 tumor suppressor.
    Article Snippet: .. Total RNA isolation was performed using NucleoSpin RNA Kit (Macherey-Nagel), following the manufacturer’s protocol. qRT-PCR with 100 ng template total RNA per reaction was performed using the Cfx96 device (Bio-Rad), and the Power SYBR Green 1-Step kit (Thermo Fisher Scientific) according to manufacturer’s protocol. ..

    Article Title: Integrated mathematical and experimental modeling uncovers enhanced EMT plasticity upon loss of the DLC1 tumor suppressor
    Article Snippet: .. Total RNA isolation was performed using NucleoSpin RNA Kit (Macherey-Nagel), following the manufacturer’s protocol. qRT-PCR with 100 ng template total RNA per reaction was performed using the Cfx96 device (Bio-Rad), and the Power SYBR Green 1-Step kit (Thermo Fisher Scientific) according to manufacturer’s protocol. ..

    SYBR Green Assay:

    Article Title: Integrated mathematical and experimental modeling uncovers enhanced EMT plasticity upon loss of the DLC1 tumor suppressor.
    Article Snippet: .. Total RNA isolation was performed using NucleoSpin RNA Kit (Macherey-Nagel), following the manufacturer’s protocol. qRT-PCR with 100 ng template total RNA per reaction was performed using the Cfx96 device (Bio-Rad), and the Power SYBR Green 1-Step kit (Thermo Fisher Scientific) according to manufacturer’s protocol. ..

    Article Title: Integrated mathematical and experimental modeling uncovers enhanced EMT plasticity upon loss of the DLC1 tumor suppressor
    Article Snippet: .. Total RNA isolation was performed using NucleoSpin RNA Kit (Macherey-Nagel), following the manufacturer’s protocol. qRT-PCR with 100 ng template total RNA per reaction was performed using the Cfx96 device (Bio-Rad), and the Power SYBR Green 1-Step kit (Thermo Fisher Scientific) according to manufacturer’s protocol. ..

    Article Title: ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells
    Article Snippet: RNA was extracted with Tri-Reagent (Sigma) and reverse transcribed to cDNA with High-Capacity cDNA Reverse Transcription Kit (Life Technologies). .. RNA levels were measured by qPCR with iTaq Universal SYBR Green Supermix (BIO-RAD) and the BioRad CFX96 device. ..

    Countercurrent Chromatography:

    Article Title: HB-EGF activates EGFR to induce reactive neural stem cells in the mouse hippocampus after seizures
    Article Snippet: Reverse transcription was done using a high-capacity reverse transcription kit (Cat#S7810; Thermo Fisher Scientific). .. A real-time quantitative polymerase chain reaction (qRT–PCR) was performed on a Bio-Rad CFX96 device (Bio-Rad), using the following primers: Hypoxanthine–guanine phosphoribosyltransferase (HPRT) forward: 5′-GTT GGG CTT ACC TCA CTG CT-3′, reverse: 5′-TCA TCG CTA ATC ACG ACG CT-3′; glyceraldehyde phosphate dehydrogenase (GAPDH) forward: 5′-CCA GTA TGA CTC CAC TCA CG-3′, reverse: 5′-GAC TCC ACG ACA TAC TCA GC-3′; EGFR forward: 5′-GCC AAC TGT ACC TAT GGA TGT-3′, reverse: 5′-GGC CCA GAG GAT TTG GAA GAA-3′; FGFR 1 forward: 5′-CCA AAC CCT GTA GCT CCC TA-3′, reverse: 5′-TGA ACT TCA CCG TCT TGG CA-3′; FGFR2 forward: 5′-CCG AAT GAAGAC CAC GAC CA-3′; reverse: 5′-TCG GCC GAA ACT GTT ACC TG-3′; metallothionein (MTH) 1 forward: 5′-TCA CCA CGA CTT CAA CGT CC-3′, reverse: 5′-CAG TTG GGG TCC ATT CCG AG-3′; MTH2 forward: 5′-GCA TCT GCA AAG AGG CTT CC-3′, reverse: 5′-AGT TGT GGA GAA CGA GTC AGG-3′; MTH3 forward: 5′-GCT GCT GGA CTG GAT ATG GA-3′; reverse: 5′-TTG CAT TTG TCC GAG CAG GT-3′. ..

    Amplification:

    Article Title: The Function of the XlnR Transcription Factor in the Filamentous Fungus Penicillium verruculosum
    Article Snippet: The Penicillium verruculosum filamentous fungus is a highly active producer of cellulolytic complex enzymes, cellobiohydrolases, endoglucanases and β-glucosidases.. Using the CRISPR/Cas9 genome editing system, previously adapted to P. verruculosum, a strain with a knockout of the xlnR gene encoding XlnR, one of the main transcription factors of filamentous fungi, has been obtained.. The transcription level of cellulolytic genes was determined by quantitative PCR for the P. verruculosum B1-221-151 strain and the new P. verruculosum ΔxlnR strain.

    Article Title: Improvement of the diagnosis of intestinal protozoa using a multiplex qPCR strategy compared to classical microscopy: a prospective study on 3,500 stool samples over 3 years
    Article Snippet: Fresh stool samples were suspended in FecalSwab medium (Copan Diagnostics, Murrieta, CA, USA), and DNA was extracted in 96-well plates using Universal Cartridges on a MICROLAB STARlet (Hamilton Company, Reno, NV, USA) following manufacturer’s instructions, and mix distribution and plate set-up were fully automated, following Seegene parameters, including negative and positive controls. .. Amplification was performed using a CFX96 device (Bio-Rad, Marnes-la-Coquette, France). .. Amplification curves were analyzed using the Seegene Viewer software (Seegene).

    Software:

    Article Title: The Function of the XlnR Transcription Factor in the Filamentous Fungus Penicillium verruculosum
    Article Snippet: The Penicillium verruculosum filamentous fungus is a highly active producer of cellulolytic complex enzymes, cellobiohydrolases, endoglucanases and β-glucosidases.. Using the CRISPR/Cas9 genome editing system, previously adapted to P. verruculosum, a strain with a knockout of the xlnR gene encoding XlnR, one of the main transcription factors of filamentous fungi, has been obtained.. The transcription level of cellulolytic genes was determined by quantitative PCR for the P. verruculosum B1-221-151 strain and the new P. verruculosum ΔxlnR strain.

    Article Title: Genomic characterization and phylogenetic evolution of the canine parvoviruses in Iranian dogs, a nationwide study: CPV evolutionary analysis in Iran.
    Article Snippet: Canine Parvo Virus 2 (CPV-2) culminated in lots of fatalities in domestic dogs since its emergence in 1978.. Mainly, it is responsible for severe hemorrhagic diarrhea, vomiting, and dehydration.. CPV-2 has three main variants known as 2a, 2b, and 2c.

    Sequencing:

    Article Title: Genomic characterization and phylogenetic evolution of the canine parvoviruses in Iranian dogs, a nationwide study: CPV evolutionary analysis in Iran.
    Article Snippet: Canine Parvo Virus 2 (CPV-2) culminated in lots of fatalities in domestic dogs since its emergence in 1978.. Mainly, it is responsible for severe hemorrhagic diarrhea, vomiting, and dehydration.. CPV-2 has three main variants known as 2a, 2b, and 2c.



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